稻瘟病菌对新型模式植物二穗短柄草的致病性研究

doi:10.3969/j.issn.1001-7216.2011.03.013

中国水稻科学

稻瘟病菌对新型模式植物二穗短柄草的致病性研究

王小艳^1,2;王教瑜^2,*;姜华² ;王艳丽²;张震²;柴荣耀²;毛雪琴²;邱海萍²;杜新法²;孙国昌^2,*

¹浙江师范大学化学与生命科学学院，浙江金华 321000； ²浙江省农业科学院植物保护与微生物研究所，浙江杭州 310021； *通讯联系人， E-mail:jiaoyuwang1@gmail.com; sungc01@sina.com

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-10 发布日期:2011-05-10

Pathogenicity of Rice Blast Fungus Magnaporthe oryzae on Brachypodium distachyon

WANG Xiao-yan^{1, 2}; WANG Jiao-yu^2,*; JIANG Hua²; WANG Yan-li²; ZHANG Zhen²; CHAI Rong-yao²; MAO Xue-qin²;QIU Hai-ping²; DU Xin-fa²; SUN Guo-chang^2,*

¹College of Chemistry and Life Sciences， Zhejiang Normal University， Jinhua 321000， China； ²Institute of Plant Protection and Microbiology， Zhejiang Academy of Agricultural Sciences, Hangzhou 310021， China； *Corresponding authors, E-mail: jiaoyuwang1@gmail.com； sungc01@sina.com

Received:1900-01-01 Revised:1900-01-01 Online:2011-05-10 Published:2011-05-10

摘要/Abstract

摘要： 建立了稻瘟病菌对二穗短柄草的接种体系，观察了稻瘟病菌在二穗短柄草上的发病过程和特点，并与在水稻和大麦上的发病情况进行了比较。在人为接种稻瘟病菌的条件下，二穗短柄草的叶片、叶鞘、茎秆、穗部均可获病。利用稻瘟病菌孢子悬浮液对二穗短柄草进行苗期活体或离体接种，都能引发典型病斑。与大麦相比，二穗短柄草叶片上病斑的出现时间及发展速度更接近水稻。同时，二穗短柄草叶片上的接种和发病条件比水稻更易控制，病斑更具一致性。另外，稻瘟病菌致病突变体在二穗短柄草叶片上致病性变化（降低或丧失）趋势与在水稻上的情况一致。显微观察及组织化学染色表明，稻瘟病菌在二穗短柄草叶片上可有效形成附着胞，侵入叶片表皮细胞，形成典型的侵染菌丝，其过程与水稻近似。因此，二穗短柄草可以作为稻瘟病菌与寄主互作的研究材料，并有望为杂草上梨孢菌的研究提供可能的模式植物以及为农作物的抗病育种提供参考。

关键词: 二穗短柄草, 稻瘟病菌, 互作, 模式植物

Abstract: An inoculation method for rice blast fungus Magnaporthe oryzae to Brachypodium distachyon was developed to investigate the infection process and symptom development in comparison with those on rice (Oryza sativa) and barley (Hordeum Vulgare). M. oryzae could infect the leaves, sheathes, stems and panicles of B. distachyon and cause blast disease. Spraying conidium suspension on either whole seedlings or leaf segments led to typical symptoms on B. distachyon. During the whole seedling inoculation, the symptoms on B. distachyon leaves developed closely resemble with rice; but the lesions on B. distachyon had better uniformity in shapes and sizes than those on rice or barley. In the leaf segments inoculation, only initiate and lowdeveloped lesions could be found on rice, while normal symptoms on B. distachyon and barley. Inoculated with lowvirulent mutants of M. oryzae, B. distachyon produced lowlevel symptoms. And the symptom level of each mutant on B. distachyon corresponded well to that on rice. In addition, typical infection processes presented on B. distachyon leaves: forming melanized appressoria, penetrating into host epiderm and then forming hyphae in epidermal cells. According to these results, B. distachyon can be used as a candidate for studying fungusplant interactions and as a probable source of disease resistance.

Key words: Brachypodium distachyon, Magnaporthe oryzae, interaction, model plant, pathogenicity

王小艳,王教瑜,姜华 ,王艳丽,张震,柴荣耀,毛雪琴,邱海萍,杜新法,孙国昌,. 稻瘟病菌对新型模式植物二穗短柄草的致病性研究[J]. 中国水稻科学 2011,25(3): 314-320 . , DOI: 10.3969/j.issn.1001-7216.2011.03.013 .

WANG Xiao-yan,WANG Jiao-yu,JIANG Hua,WANG Yan-li,ZHANG Zhen,CHAI Rong-yao,MAO Xue-qin,QIU Hai-ping,DU Xin-fa,SUN Guo-chang. Pathogenicity of Rice Blast Fungus Magnaporthe oryzae on Brachypodium distachyon [J]. Chinese Journal of Rice Science 2011,25(3): 314-320 ., DOI: 10.3969/j.issn.1001-7216.2011.03.013 .

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稻瘟病菌对新型模式植物二穗短柄草的致病性研究

Pathogenicity of Rice Blast Fungus Magnaporthe oryzae on Brachypodium distachyon

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